Detection of Thalidomide Embryotoxicity by In Vitro Embryotoxicity Testing Based on Human iPS Cells

In Vitro Embryotoxicity Testing of Mercury Vapour by Differentiation of ES-D3 Cells

The embryonic stem cell test (EST) is an in vitro assay that has been developed to assess the embryotoxic potential of chemicals and biomaterials. It is based on the capacity of the mouse embryonic stem cell line D3 (ES-D3 cells) to differentiate into contracting myocardial cells. However, the EST method cannot fully test the embryotoxicity of mercury vapor. We tried to apply the rotary culture...

Development of technique for in vitro embryotoxicity of dental biomaterials

The Embryonic Stem Cell Test (EST) developed in Germany in 1997 is known as a screening test method capable of predicting the presence of unknown chemicals influencing normal human development. Firstly, we investigated the embryotoxicity of 24 types of monomer including dental monomers and dental alloy-component metal elements using this test. Monomers including Bis-GMA contained in base resin ...

In vitro embryotoxicity assessment with dental restorative materials.

OBJECTIVES Resin (co)monomers may be released from restorative dental materials and can diffuse into the tooth pulp or the gingiva, and can reach the saliva and the circulating blood. Genotoxic potential of some dental composite components has been clearly documented. The genotoxic effects of xenobiotics can represent a possible step in tumor initiation and/or embryotoxicity/teratogenesis. A mo...

Chloroquine embryotoxicity in the postimplantation rat conceptus in vitro.

The embryotoxicity of the antimalarial drug chloroquine (CQ) was evaluated in vitro using the rat whole embryo culture system. CQ was found to be embryotoxic and dysmorphogenic when added directly to the culture media containing gestational day (GD) 10 rat conceptuses. Twenty-six-hr exposure to CQ elicited dose-related decreases in embryonic crown-rump length, protein and DNA contents and incre...

Embryotoxicity of 4-deoxynivalenol (vomitoxin) in mice.